ABSTRACT
In this study, we investigated the chemical profile and effects of RW0117 (Artemisia argyi 65 .5 % ethanol extract) on gastric lesions in rats. We optimized and validated a method to obtain the chemical profile of RW0117. We then investigated the antioxidant and anti-inflammatory effects in vivo, and the protective effects on gastric lesions in vivo. The IC50 of 2,2-diphenyl-1-picrylhydrazyl free radical scavenging considering the antioxidant effects of RW0117 was 166.55 μg/mL, and the IC50 of nitric oxide scavenging considering the antiinflammatory effects was 41.16 μg/mL. Oral administration of RW0117 at lower concentrations (25, 50, 100 mg/ kg) had similar or greater effects than the daily intake conversion concentration (115mg/kg) of a health functional food (Avexol® ) in the acetic acid-induced ulcer and the ethanol-induced gastric injury rat models. In addition, oral administration of RW0117 increased the expression of prostaglandin E2 , which enhances the protective effect in the gastric mucosa in the ethanol-induced gastric injury rat model. These results suggest that RW0117 may have potential therapeutic uses in the protection of the gastric mucosa.
ABSTRACT
In this study, we investigated the chemical profile and effects of RW0117 (Artemisia argyi 65 .5 % ethanol extract) on gastric lesions in rats. We optimized and validated a method to obtain the chemical profile of RW0117. We then investigated the antioxidant and anti-inflammatory effects in vivo, and the protective effects on gastric lesions in vivo. The IC50 of 2,2-diphenyl-1-picrylhydrazyl free radical scavenging considering the antioxidant effects of RW0117 was 166.55 μg/mL, and the IC50 of nitric oxide scavenging considering the antiinflammatory effects was 41.16 μg/mL. Oral administration of RW0117 at lower concentrations (25, 50, 100 mg/ kg) had similar or greater effects than the daily intake conversion concentration (115mg/kg) of a health functional food (Avexol® ) in the acetic acid-induced ulcer and the ethanol-induced gastric injury rat models. In addition, oral administration of RW0117 increased the expression of prostaglandin E2 , which enhances the protective effect in the gastric mucosa in the ethanol-induced gastric injury rat model. These results suggest that RW0117 may have potential therapeutic uses in the protection of the gastric mucosa.